浠婂ぉ鏄細

瀹為獙瀹よ祫璁綉

鍗庡ぇ鏅洪€犲畬鎴?浜跨編鍏冮杞嫙璧勶紝鍔犲己鍥戒骇鏂板瀷鍩哄洜娴嬪簭浠爺鍙?

瀹為獙瀹よ祫璁綉鏃堕棿:2019-05-17 鐐瑰嚮: 娆?/span> 鐧惧害鎼滅储

銆愬璇汇€?/strong>娴嬪簭涓浗2019骞?鏈?鏃ユ秷鎭紝鍗庡ぇ鏅洪€犻杞嫙璧勮妯¤秴杩?2浜跨編鍏冿紝鍒涢€?018骞村害鍥藉唴鍩哄洜棰嗗煙鏈€澶у嫙璧勯噾棰濄€傝杞嫙璧勬姇璧勪汉涓轰腑淇¢噾鐭炽€佹澗绂捐祫鏈€佷笢璇佽祫鏈瓑澶氬鐭ュ悕鏈烘瀯銆?鏍稿績鎶€鏈疍NBSEQ TM 鎵撶牬涓婃父鍨勬柇 鍩哄洜娴嬪簭浠槸鍩哄洜娴嬪簭浜т笟鐨勪笂娓稿钩鍙帮紝涔熸槸鍩哄洜妫€娴嬨€佸熀鍥犵紪杈戙€佸熀鍥犲悎鎴愪骇涓氫腑鐨勫喅瀹氭€ц澶?.....
TAG鏍囩锛?鐮斿彂 鍩哄洜娴嬪簭浠?/a> 鍗庡ぇ鏅洪€?/a> 棣栬疆鍕熻祫 鍥戒骇鏂板瀷
鍗庡ぇ鏅洪€犲畬鎴?浜跨編鍏冮杞嫙璧勶紝鍔犲己鍥戒骇鏂板瀷鍩哄洜娴嬪簭浠爺鍙?

娴嬪簭涓浗2019骞?鏈?鏃ユ秷鎭紝鍗庡ぇ鏅洪€犻杞嫙璧勮妯¤秴杩?/span>2浜跨編鍏冿紝鍒涢€?018骞村害鍥藉唴鍩哄洜棰嗗煙鏈€澶у嫙璧勯噾棰濄€傝杞嫙璧勬姇璧勪汉涓轰腑淇¢噾鐭炽€佹澗绂捐祫鏈€佷笢璇佽祫鏈瓑澶氬鐭ュ悕鏈烘瀯銆?/p>

鏍稿績鎶€鏈疍NBSEQTM鎵撶牬涓婃父鍨勬柇

鍩哄洜娴嬪簭浠槸鍩哄洜娴嬪簭浜т笟鐨勪笂娓稿钩鍙帮紝涔熸槸鍩哄洜妫€娴嬨€佸熀鍥犵紪杈戙€佸熀鍥犲悎鎴愪骇涓氫腑鐨勫喅瀹氭€ц澶囥€傞暱涔呬互鏉ワ紝鍩哄洜娴嬪簭涓婃父鎶€鏈灏戞暟澶栧浗浼佷笟鎶婃寔锛屽苟鏈夌潃涓ュ瘑鐨勪笓鍒╀繚鎶ゃ€?span style="font-weight: 700; border: 0px; margin: 0px; padding: 0px;">涓烘墦鐮村瀯鏂眬闈紝鍗庡ぇ鏅洪€犻€氳繃鎶€鏈紩杩涗笌鍒涙柊锛岄噰鍙栦笉鍚岀殑鎶€鏈矾绾匡紝鎴愬姛寮€鍙戜簡鍏堣繘鐨凞NBSEQTM娴嬪簭鎶€鏈€傚崕澶ф櫤閫犺兘澶熸垚鍔熷畬鎴愰杞嫙璧勶紝鍦ㄤ竴瀹氱▼搴︿笂涔熷緱鐩婁簬鍏禗NBSEQTM娴嬪簭鎶€鏈€?/p>

鎹簡瑙o紝DNBSEQTM娴嬪簭鎶€鏈富瑕佸寘鎷細DNA鍗曢摼鐜寲鍜孌NB鍒跺銆佽鍒欓樀鍒楄姱鐗囥€丏NB鍔犺浇銆乧PAS銆佸弻绔祴搴忔妧鏈€佺⒈鍩鸿瘑鍒畻娉曠瓑銆傚湪DNBSEQTM娴嬪簭鎶€鏈腑锛屼华鍣ㄦ皵娑茬郴缁熶細鍏堝皢DNA绾崇背鐞冩车鍏ュ埌瑙勫垯闃靛垪鑺墖骞跺姞浠ュ浐瀹氾紝鐒跺悗娉靛叆娴嬪簭妯℃澘鍙婃祴搴忚瘯鍓傦紝鏈€缁堣幏寰楀緟娴嬫牱鏈殑纰卞熀搴忓垪淇℃伅銆傜洰鍓嶏紝cPAS宸茬粡骞挎硾琚簲鐢ㄤ簬鍗庡ぇ鏅洪€犲紑鍙戠殑BGISEQ-500銆丅GISEQ-50銆丮GISEQ-200銆丮GISEQ-2000浠ュ強MGISEQ-T7绛夊瀷鍙峰熀鍥犳祴搴忎华涓€?/p>

鍗庡ぇ鏅洪€犲畬鎴?浜跨編鍏冮杞嫙璧勶紝鍔犲己鍥戒骇鏂板瀷鍩哄洜娴嬪簭浠爺鍙?

鍥?. DNB鍒跺绀烘剰鍥?/p>

鍊煎緱涓€鎻愮殑鏄紝涓庡叾浠栫幇鏈夌殑娴嬪簭鎶€鏈浉姣旓紝DNBSEQTM娴嬪簭鎶€鏈粨鍚堜簡DNB鐨勪綆閿欒绉疮鍜岃鍒欓樀鍒楄姱鐗囩殑楂樹俊鍙峰瘑搴︼紝鑳藉鎻愰珮妫€娴嬬殑鍑嗙‘鎬у苟瀹炵幇鏇撮珮鏁堢殑澶ц妯″苟琛屾祴搴忋€傚鍦╓GS/WES绛夊叿浣撳簲鐢ㄤ腑锛岃鎶€鏈骇鍑烘暟鎹噸澶嶅簭鍒楃巼浣庯紝鍙噺灏戞暟鎹氮璐广€傚熀浜嶱CR-Free鐨勫缓搴撴柟娉曪紝杩樺叿鏈夋洿濂界殑SNP鍜孖nDel鍑嗙‘鎬с€傛澶栵紝BGISEQ/MGISEQ绯诲垪娴嬪簭骞冲彴鐨勬爣绛捐烦璺冨皯锛屼篃鍙湁鏁堥檷浣庢牱鏈敊璇垎閰嶇殑“寮犲啝鏉庢埓”闂銆?/p>

鍗庡ぇ鏅洪€犲畬鎴?浜跨編鍏冮杞嫙璧勶紝鍔犲己鍥戒骇鏂板瀷鍩哄洜娴嬪簭浠爺鍙?

鍥?. DNB鍔犺浇杩囩▼绀烘剰鍥?/p>

鍙﹀锛岄拡瀵逛笟鍐呮祴搴忔櫘閬嶈闀胯緝鐭殑缂虹偣锛?018骞村崕澶ф櫤閫犺繕鍙戝竷浜嗘柊涓€浠i暱鐗囨寤哄簱鎶€鏈?mdash;—stLFR锛堝崟绠℃棤鍒嗛殧闀跨墖娈佃鍙栵級銆傜洰鍓嶏紝鍩轰簬璇ユ妧鏈浆鍖栫殑璇曞墏鐩掍骇鍝佸凡姝e紡涓婂競銆傚崕澶ф櫤閫犺繕浠嬬粛锛岀浉姣斾紶缁焀GS鏂囧簱鍜屽叾浠栧悓绫讳骇鍝侊紝stLFR鏂囧簱浠呴渶1ng DNA涓旀棤闇€棰勬墿澧炲嵆鍙幏寰楅珮璐ㄩ噺鐨勫叏鍩哄洜缁勬祴搴忔枃搴擄紝浜屽€嶄綋瀹氱浉鍖哄潡N50鍊煎彲杈?0Mb锛岃兘澶熸湁鏁堟鍑哄€掍綅銆佸紓浣嶃€佸垹闄ょ瓑缁撴瀯鍙樺紓锛屽疄鐜板父瑙勬墜娈垫棤娉曞疄鐜扮殑缁撴瀯鍙樺紓妫€娴嬶紝骞惰揪鍒扮浉鍚屾繁搴︾殑甯歌鍏ㄥ熀鍥犵粍娴嬪簭鏂囧簱琛ㄧ幇鐨勬渶浣虫按骞炽€傛澶栵紝璇ヤ骇鍝佸崟绠℃棤鍒嗛殧鍙嶅簲锛屾棤闇€寰噺绉绘恫璁惧鎴栧鏉傚井娴佹帶绯荤粺锛涘叾鍏ㄩ儴鍙嶅簲鍦ㄧ鐝犱笂瀹屾垚锛屾槗浜庨珮閫氶噺鑷姩鍖栧缓搴擄紝鍥犳澶у箙闄嶄綆浜嗛暱鐗囨鏂囧簱鏋勫缓鐨勫鏉傜▼搴﹀拰鎴愭湰銆?/p>

鍗庡ぇ鏅洪€犲畬鎴?浜跨編鍏冮杞嫙璧勶紝鍔犲己鍥戒骇鏂板瀷鍩哄洜娴嬪簭浠爺鍙?

鍥?. MGIEasy stLFR鏂囧簱鏋勫缓璇曞墏鐩?/p>

鍥為【鍗庡ぇ鏅洪€犳妧鏈彂灞曞巻绋嬶紝浠庢渶鍒濇敹璐編鍥紺omplete Genomics鍏徃鑾峰彇鏍稿績鎶€鏈紝瀹炵幇鍩哄洜娴嬪簭宸ュ叿鐨勮嚜涓诲彲鎺э紝鍒扮湡姝f嫢鏈夎嚜宸辩殑鏍稿績涓撳埄锛屽崕澶ф櫤閫犳閫愭鍦ㄥ熀鍥犳祴搴忎骇涓氫笂娓告墦鐮村浼佺殑甯傚満鍨勬柇銆?/p>

鎵撻€犵敓鍛芥暟瀛楀寲鍏ㄦ柟浣嶄骇鍝?/span>

杩戝勾鏉ワ紝闅忕潃鍩哄洜娴嬪簭鎶€鏈殑椋為€熷彂灞曪紝鍩哄洜娴嬪簭鍏ㄧ悆甯傚満姝d粠绉戠爺璧板悜涓村簥锛屽競鍦鸿妯′篃鐢辨杩堝叆楂橀€熸垚闀挎湡銆傛湁鐮旂┒棰勪及锛屽叏鐞冩祴搴忎骇涓氬競鍦鸿妯″湪2022骞村皢杈惧埌124.5浜跨編鍏冿紝鍏朵腑浜氬お鍦板尯灏嗚揪鍒?3.9浜跨編鍏冦€傚埌2020骞达紝涓浗鍩哄洜娴嬪簭琛屼笟甯傚満瑙勬ā灏嗚揪鍒?8浜垮厓宸﹀彸锛屾湭鏉ュ嚑骞村唴鍥藉唴鍩哄洜娴嬪簭甯傚満闇€姹傚皢绋虫閲婃斁锛屽競鍦鸿妯″皢淇濇寔鑹ソ澧炲娍銆?/p>

鍩哄洜浜т笟甯傚満姝e紩棰嗕笘鐣岀鎶€娴疆鐨?ldquo;鏂拌禌閬?rdquo;銆傚崕澶ф櫤閫犱箣鎵€浠ヨ璁や负鏄繖涓€“鏂拌禌閬?rdquo;鐨勯噸瑕佹妧鏈瀯寤鸿€呬箣涓€锛?span style="font-weight: 700; border: 0px; margin: 0px; padding: 0px;">鍏抽敭鍦ㄤ簬鍏跺湪涓婃父娴嬪簭鎶€鏈腑鐨勬寔缁姇鍏ュ拰鐮村眬璧惰秴銆傛埅鑷崇洰鍓嶏紝璇ュ叕鍙稿凡绱鎶曞叆绾?0浜垮厓鐮斿彂缁忚垂锛屽疄鐜版祴搴忔妧鏈簮澶村紡涓撳埄甯冨眬锛岀敵璇锋妧鏈笓鍒?80浣欓」锛屽叾涓璓CT涓撳埄80浣欓」銆?/p>

鑳岄潬鍗庡ぇ闆嗗洟鐨勫熀鍥犵敓鎬侀摼锛屼緷鎵樺叾鑷湁鎶€鏈矾绾垮拰涓嶆柇寮哄寲鐨勬妧鏈紭鍔匡紝鑷?015骞翠互鏉ワ紝鍗庡ぇ鏅洪€犲凡鎺ㄥ嚭澶氭涓嶅悓閫氶噺鐨勬祴搴忎华銆傚叏绯诲垪鐨勪骇鍝佸竷灞€浠ュ強娑电洊灏忓瀷鍖栥€侀珮閫氶噺澶氭牱鍖栭渶姹傦紝姝f垚涓鸿鍏徃鎷撳睍鍏ㄧ悆甯傚満鐨勪竴澶у姪鍔涖€?/p>

浣滀负涓诲姏鏈哄瀷锛屽崕澶ф櫤閫犳帹鍑虹殑MGISEQ-2000鍏ㄩ潰鍨嬪熀鍥犳祴搴忎华鍦ㄥ弻鑺墖鐙珛杩愯骞冲彴鐨勫熀纭€涓婏紝鍙悓鏃舵敮鎸佷袱绉嶄笉鍚岃鏍肩殑鑺墖銆傚叾鍗曟杩愯鑳藉瀹屾垚鏈€澶?080Gb鐨勪笅鏈烘暟鎹紝鍦≒E100璇婚暱妯″紡涓嬫弧璐熻嵎杩愯浠呴渶48灏忔椂锛屼竴骞寸殑杩愯閫氶噺鐩稿綋浜?000涓互涓婁汉鍏ㄥ熀鍥犵粍鏁版嵁閲忋€備笌涔嬪悓鏈熷彂甯冪殑MGISEQ-200鐗圭偣鏄洿涓烘柟渚跨伒娲伙紝鍦≒E100璇婚暱妯″紡涓嬫弧璐熻嵎杩愯鍙骇鐢?0Gb鐨勪笅鏈烘暟鎹紝浠呴渶涓嶅埌48灏忔椂锛屽钩鍧囦竴澶╄兘瀹屾垚24涓偪鐦ゆ牱鏈殑蹇€熸娴嬨€傞櫎姝や箣澶栵紝璇ュ叕鍙镐簬2018骞?0鏈堝彂甯冪殑MGISEQ-T7鍒欐槸鍏朵骇鍝佸竷灞€涓殑“鏄庢槦”鏈哄瀷銆傚€煎緱涓€鎻愮殑鏄紝MGISEQ-T7杩樻槸鐩墠鍏ㄧ悆鏃ョ敓浜ц兘鍔涙渶寮虹殑鍩哄洜娴嬪簭浠紝鍗曟棩鏁版嵁浜ч噺鍙揪6Tb锛孭E150娴嬪簭浠呴渶24灏忔椂锛屼笖姣廏b娴嬪簭璐圭敤浠呬负5缇庨噾锛岃繖涔熸槸鐩墠鍏ㄧ悆鏈€浣庣殑娴嬪簭鎴愭湰銆?/p>

鍗庡ぇ鏅洪€犲畬鎴?浜跨編鍏冮杞嫙璧勶紝鍔犲己鍥戒骇鏂板瀷鍩哄洜娴嬪簭浠爺鍙?

鍥?. MGISEQ-2000锛堝乏锛夈€丮GISEQ-200锛堜腑锛夈€丮GISEQ-T7锛堝彸锛?/p>

鎹崕澶ф櫤閫犻€忛湶锛岀洰鍓嶈鍏徃娴嬪簭浠叏鐞冭鏈洪噺宸茶秴杩?100鍙帮紝閬嶅竷16涓浗瀹跺強鍦板尯锛岀敤鎴疯秴300瀹讹紝绱鏁版嵁瓒呰繃20Pb锛屽彂琛ㄩ珮姘村钩绉戠爺璁烘枃300浣欑瘒銆傝繎鏈燂紝鍗庡ぇ鏅洪€犺繕鑱斿悎涓浗涓尰绉戝闄腑鑽爺绌舵墍鐮斿埗骞跺彂甯冧簡涓崏鑽疍NA鏉″舰鐮侀珮閫氶噺鍩哄洜娴嬪簭涓€浣撴満銆傝浠櫒鏄?span style="font-weight: 700; border: 0px; margin: 0px; padding: 0px;">鍏ㄧ悆棣栧垱涓崏鑽熀鍥犳祴搴忔櫤鑳介壌瀹氫华锛屽彲绮惧噯銆佸揩閫熴€佺畝鏄撱€佺ǔ瀹氶壌瀹氫腑鑽夎嵂鏍峰搧銆傝繖涓€涓炬帾涔熻繘涓€姝ユ縺鍙戜簡鍥藉唴鍏朵粬缁嗗垎甯傚満鐨勪骇鍝佸畾鍒堕渶姹傘€?/p>

鍦ㄥ竷灞€鍏ㄧ郴鍒楁祴搴忎华浜у搧鐨勫悓鏃讹紝鍗庡ぇ鏅洪€犺繕鎴愬姛鐮斿彂涓庝箣鐩稿尮閰嶇殑娴嬪簭璇曞墏銆佹潗鏂欍€佺敓鍖栧弽搴斾綋绯荤瓑涓€绯诲垪鐨勬牳蹇冩妧鏈紝甯冨眬鍏ㄧ郴鍒椾骇鍝併€傞櫎stLFR寤哄簱璇曞墏鐩掑锛?span style="font-weight: 700; border: 0px; margin: 0px; padding: 0px;">鍗庡ぇ鏅洪€犺繕鑷富寮€鍙戜簡纰卞熀璇嗗埆绠楁硶Sub-pixel Registration锛屼娇鍥惧儚閰嶅噯绮剧‘搴﹁揪鍒颁簡浜氬儚绱犵骇鍒紝澶уぇ鎻愰珮浜嗙⒈鍩鸿瘑鍒殑鍑嗙‘搴︺€?/span>閫氳繃GPU鍔犻€熺殑鏂规硶锛屽湪鏇撮珮绮惧害鐨勭畻娉曟潯浠朵笅瀹炵幇浜?00鍊嶄互涓婄殑鍔犻€熴€傚湪鎻愰珮璇嗗埆鍑嗙‘鐜囩殑鍚屾椂瀹炵幇浜嗗浘鍍忓鐞嗗拰纰卞熀璇嗗埆鐨勫疄鏃跺寲锛屾暟鎹鐞嗛€熷害澶勪簬棰嗗厛姘村钩銆傛澶栵紝鍗庡ぇ鏅洪€犺繕鍙戞槑浜嗚崸鍏変笌纰卞熀缁撳悎鐨勬柊鏂规硶——鐢ㄦ姉浣撲綔涓哄獟浠嬶紝杩涗竴姝ユ彁楂樿闀垮強娴嬪簭鐨勫噯纭€с€?/p>

鍗庡ぇ鏅洪€犲畬鎴?浜跨編鍏冮杞嫙璧勶紝鍔犲己鍥戒骇鏂板瀷鍩哄洜娴嬪簭浠爺鍙?

鍥?. 纰卞熀璇嗗埆绠楁硶娴佺▼

鎺ㄥ姩鍩哄洜娴嬪簭浜т笟鍋ュ悍鍙戝睍

鍦ㄧ幇鏈夌殑楂橀€氶噺娴嬪簭鎶€鏈笅锛屼汉浠鍚勭鐤剧梾鐨勮鐭ュ拰闃叉帶宸插彇寰楅暱瓒宠繘灞曪紝浣嗗湪鍩哄洜璁ょ煡鏂归潰杩樻槸闈复寰堝鎶€鏈寫鎴樸€傚洜姝わ紝鏈潵瀵圭柧鐥呯殑璁ょ煡浠嶉渶鎶€鏈笌璁惧鐨勪笉鏂洿鏂般€?/p>

鏀剧溂鍏ㄧ悆锛屽熀鍥犳祴搴忎华甯傚満鐨勫皝闂牸灞€锛屼护琛屼笟涓嬫父鍘傚晢鍙戝睍闅句互鑾峰緱鍏呭垎鐨勬妧鏈€夋嫨绌洪棿鍜屾湇鍔℃敮鎸佸姏搴︺€傜獊鐮村熀鍥犳祴搴忎笂娓告牳蹇冩妧鏈紝鍙皳椤哄娍鍥犳椂銆傚崕澶ф櫤閫犻€氳繃鎶€鏈紩杩涘拰鑷富鍒涙柊锛岄噰鍙栦笉鍚岀殑鎶€鏈矾绾匡紝姝e悜鏇村揩銆佹洿鍑嗙殑娴嬪簭鎶€鏈繄杩涳紝涓轰腑鍥藉熀鍥犳祴搴忎骇涓氬仴搴峰彂灞曟彁渚涘己鍔茬殑鍔ㄥ姏銆?/p>

鍦ㄦ祴搴忔妧鏈疄鐜板集閬撹秴杞﹀悗锛屽崕澶ф櫤閫犲啀鎺ュ啀鍘夛紝渚濋潬寮哄ぇ鐨勭爺鍙戝洟闃熸垚鍔熷紑鍙戜笌涔嬮厤濂楃殑鑷姩鍖栬澶囧強鑰楁潗銆?/span>浠庢妧鏈彈鍥藉浼佷笟鎺h倶锛屽埌瀹炵幇鍏ㄧ郴鍒椾骇鍝佸竷灞€锛屽湪娴嬪簭浠瓑涓婃父鎶€鏈骇鍝佺殑鐮斿埗鐢熶骇涓婏紝涓浗浼佷笟寮€濮嬪喘闇插ご瑙掋€傚崕澶ф櫤閫犻€氳繃鑷富鐮斿彂閫愭鎵撶牬澶栦紒鐨勫競鍦哄瀯鏂紝瀹炵幇浜嗘垜鍥藉熀鍥犵鎶€甯冨眬浜т笟涓婃父鐨勭獊鐮达紝寮ヨˉ浜嗚棰嗗煙鐨勭┖鐧姐€?/p>

鍩哄洜娴嬪簭闄や簡鍦ㄧ鐮旈鍩熷ぇ瑙勬ā搴旂敤澶栵紝鍦ㄤ复搴婂尰瀛﹂鍩熶篃鍙戞尌鐫€閲嶈浣滅敤锛屽鏁翠釜鐢熷懡绉戝棰嗗煙鐨勫彂灞曟湁鐫€娣辫繙褰卞搷銆備负淇冭繘鍩哄洜娴嬪簭浜т笟鐨勫仴搴峰彂灞曪紝鐩墠锛?span style="font-weight: 700; border: 0px; margin: 0px; padding: 0px;">鍗庡ぇ鏅洪€犳鍩轰簬鑷富鐮斿彂鐨勯鍏堟祴搴忓钩鍙帮紝涓庝笂涓嬫父鍏卞缓绋冲仴銆佸紑鏀剧殑浜т笟鐢熸€侊紝鏀瑰彉琛屼笟鍥烘湁灏侀棴鏍煎眬銆?/span>

2018骞?鏈堬紝鍗庡ぇ鏅洪€犲湪涓浗棣栨鍚姩娴嬪簭鑰呰鍒掞紝鏃ㄥ湪鍗忓姪琛屼笟鍐呴渶瑕佹祴搴忔暟鎹骇鍑虹殑浼佷笟鍜岀鐮旀満鏋勫疄鐜拌嚜涓绘祴搴忓钩鍙板缓璁撅紝鎻愪緵鍖呮嫭璇曞墏銆佸煿璁強搴旂敤鎸囧鍦ㄥ唴鐨勫叏鏂逛綅鎶€鏈敮鎸侊紝榧撳姳鍩哄洜缁勫鎶€鏈垱鏂板拰浜т笟鍙戝睍銆傛嵁鎮夛紝璇ヨ鍒掑湪鍥藉唴鍙栧緱浜嗚壇濂界殑鍙嶅搷锛屽凡鏈夎繎10瀹跺熀鍥犺涓氭満鏋勬垚涓鸿繖涓€鑱旂洘鐢熸€佷紮浼淬€傚湪涓浗甯傚満涔嬪锛屽崕澶ф櫤閫犺繕鐩哥户鍚姩浜嗗叏鐞冨悇涓昏甯傚満鐨勬祴搴忚€呰鍒掞紝鍖呮嫭娆ф床鍦板尯娴嬪簭鑰呰鍒掋€佷簹澶湴鍖烘祴搴忚€呰鍒掋€傚湪鍏ㄧ悆鐨勫熀鍥犳祴搴忓競鍦哄彂鍑轰簡涓浗鑷繁鐙壒鐨勫0闊炽€?/p>

缁?璇?/span>

浣滀负涓浗鍞竴銆佸叏鐞冧粎涓夊鑳藉鑷富鐮斿彂骞堕噺浜т复搴婇珮閫氶噺鍩哄洜娴嬪簭浠殑鏄庢槦鍏徃锛屽崕澶ф櫤閫犻杞嫙璧勭殑瀹屾垚锛屾爣蹇楃潃鍏剁爺鍙戣兘鍔涖€佸浗闄呭寲鑳藉姏鍙楀埌浜嗚鍙紝鏈夊姪浜庝腑鍥界敓鍛界瀛﹀拰浜т笟楂樼瑁呭鍒堕€犲疄鐜颁粠璺熻釜鍒板紩棰嗙殑璺ㄨ秺銆傚浜庢湰娆″嫙璧勶紝鍗庡ぇ鏅洪€犲皢閫氳繃鏈鍕熻祫杩涗竴姝ュ姞寮烘柊鍨嬪熀鍥犳祴搴忎华鐨勭爺鍙戙€佺敓浜у伐浣滐紝鎵撻€犲洿缁曠敓鍛芥暟瀛楀寲鐨勫叏鏂逛綅浜у搧甯冨眬锛屽苟鑷村姏浜庡熀鍥犳祴搴忎骇涓氱殑甯傚満寮€鎷撳拰鐢熸€佸缓璁俱€?/span>

鐩镐俊鍦ㄦ湭鏉ョ殑鍩哄洜娴嬪簭甯傚満锛屾垜浠皢鐪嬪埌瓒婃潵瓒婂涓浗鏅洪€犵殑鍩哄洜娴嬪簭鎶€鏈拰浜у搧銆傚悓鏃讹紝涔熺浉淇′竴涓紑鏀惧叡璧㈢殑浜т笟鐢熸€佸湀锛屽皢鍔╁姏涓浗鍩哄洜浜т笟閫氳繃鍗忓悓鍒涙柊锛屾洿蹇繘鍏ュ叏鐞冨競鍦猴紝鍔犻€熷疄鐜扮敱鍙備笌銆佹帴杞ㄣ€佸悓姝ュ埌寮曢鐨勮法瓒婂彂灞曘€?/p> 锛堟湰鏂囨潵婧愶細娴嬪簭涓浗 锛?

(璐d换缂栬緫锛氬瓙璞?

寮曠敤鍦板潃锛?a href="#" target=_blank>

TAG鏍囩锛?鐮斿彂 鍩哄洜娴嬪簭浠?/a> 鍗庡ぇ鏅洪€?/a> 棣栬疆鍕熻祫 鍥戒骇鏂板瀷
椤朵竴涓?/div>
(0)
0%
韪╀竴涓?/div>
(0)
0%
鍏嶈矗澹版槑锛?/strong> 闄ゆ爣鏄庛€婂疄楠屽璧勮缃戙€嬪師鍒涘锛屾湰缃戦儴鍒嗘枃绔犺浆杞借嚜鍏跺畠濯掍綋锛岃浆杞界洰鐨勫湪浜庝紶閫掓洿澶氫俊鎭紝 骞朵笉浠h〃鏈綉璧炲悓鍏惰鐐瑰拰瀵瑰叾鐪熷疄鎬ц礋璐o紝涓斾笉鎵挎媴姝ょ被浣滃搧渚垫潈琛屼负鐨勭洿鎺ヨ矗浠诲強杩炲甫璐d换銆? 濡傚叾浠栧獟浣撱€佺綉绔欐垨涓汉浠庢湰缃戜笅杞戒娇鐢紝鑷礋鐗堟潈绛夋硶寰嬭矗浠汇€傚娑夊強浣滃搧鍐呭銆佺増鏉冨拰鍏跺畠闂锛? 璇峰湪30鏃ュ唴涓庢湰缃戣仈绯伙紝鎴戜滑灏嗗湪绗竴鏃堕棿鍒犻櫎鍐呭锛?
------鍒嗛殧绾?---------------------------
鍙戣〃璇勮
璇疯嚜瑙夐伒瀹堜簰鑱旂綉鐩稿叧鐨勬斂绛栨硶瑙勶紝涓ョ鍙戝竷鑹叉儏銆佹毚鍔涖€佸弽鍔ㄧ殑瑷€璁恒€?/small>
璇勪环:
琛ㄦ儏:
鏈€鏂拌瘎璁?/strong> 杩涘叆璇︾粏璇勮椤?gt;>