浠婂ぉ鏄細

瀹為獙瀹よ祫璁綉

鐮旂┒姣旇緝浜嗙敤浜庡崟缁嗚優RNA娴嬪簭鐨勬枃搴撳埗澶囧拰娴嬪簭骞冲彴

瀹為獙瀹よ祫璁綉鏃堕棿:2019-04-10 鐐瑰嚮: 娆?/span> 鐧惧害鎼滅储

銆愬璇汇€?/strong>涓归害鍗楅儴澶у锛屾儬搴锋鏍肩爺绌舵墍鍜孊GI鐨勭爺绌朵汉鍛樹粖澶╁湪鍩哄洜缁勭敓鐗╁鏉傚織涓婂彂琛ㄤ簡涓€椤圭爺绌讹紝姣旇緝浜嗙敤浜庡崟缁嗚優RNA娴嬪簭(scRNA-seq)鐨勬枃搴撳埗澶囧拰娴嬪簭骞冲彴銆?鍗曠粏鑳炶浆褰曠粍瀛?鍗硈cRNA-seq)鏄笅涓€浠f祴搴忔柟娉曪紝鍏跺悓鏃舵祴閲忓崟涓粏鑳炰腑鏁板崈涓熀鍥犵殑淇′娇RNA娴撳害(鐢盌NA /鍩哄洜缁?閬椾紶钃濆浘缂栫爜)銆傝繖浣跨爺绌朵汉鍛樿兘澶熻幏寰楅珮鍒?.....
TAG鏍囩锛?鍗曠粏鑳濺NA娴嬪簭 鏂囧簱鍒跺 娴嬪簭骞冲彴

涓归害鍗楅儴澶у锛屾儬搴锋鏍肩爺绌舵墍鍜孊GI鐨勭爺绌朵汉鍛樹粖澶╁湪鍩哄洜缁勭敓鐗╁鏉傚織涓婂彂琛ㄤ簡涓€椤圭爺绌讹紝姣旇緝浜嗙敤浜庡崟缁嗚優RNA娴嬪簭(scRNA-seq)鐨勬枃搴撳埗澶囧拰娴嬪簭骞冲彴銆?/p>

鐮旂┒姣旇緝浜嗙敤浜庡崟缁嗚優RNA娴嬪簭鐨勬枃搴撳埗澶囧拰娴嬪簭骞冲彴

鍗曠粏鑳炶浆褰曠粍瀛?鍗硈cRNA-seq)鏄笅涓€浠f祴搴忔柟娉曪紝鍏跺悓鏃舵祴閲忓崟涓粏鑳炰腑鏁板崈涓熀鍥犵殑淇′娇RNA娴撳害(鐢盌NA /鍩哄洜缁?閬椾紶钃濆浘缂栫爜)銆傝繖浣跨爺绌朵汉鍛樿兘澶熻幏寰楅珮鍒嗚鲸鐜囩殑缁嗚優瑙嗗浘锛屼互瑙e紑寮傝川缁嗚優缇ゅ苟鏇村ソ鍦颁簡瑙d綋鍐呯殑涓綋缁嗚優鍔熻兘銆傚敖绠″瓨鍦ㄥ嚑绉嶅崟缁嗚優鏂规锛屼絾浼犵粺涓婁娇鐢↖llumina鎶€鏈拰娴嬪簭骞冲彴杩涜娴嬪簭銆?/p>

浣滆€呭湪姝よ繘琛屼簡浼犵粺Illumina骞冲彴涓庡崟缁嗚優杞綍缁勫鐨勬浛浠GISEQ-500鐭搴忓垪骞冲彴鐨勯娆℃瘮杈冦€備綔鑰呬娇鐢ㄤ袱绉嶄笉鍚岀殑鏂规(SMARTer鍜孲mart-seq2)閫氳繃scRNA-seq鍒嗘瀽浜?68涓崟涓粏鑳烇紝浜х敓浜?297涓崟缁嗚優鏂囧簱锛岀敤浜嶪llumina HiSeq鍜孊GISEQ-500骞冲彴鐨勬祴搴忋€傞€氳繃浣跨敤涓ょ涓嶅悓鐨勭粏鑳炵被鍨?浜虹被姘哥敓鍖栫櫧琛€鐥呯粏鑳?#39;K562'鍜屽皬榧犺儦鑳庡共缁嗚優'mESCs')鍜屾幒鍏ュ悎鎴怰NA鎺у埗搴忓垪锛屼綔鑰呭娴嬪簭骞冲彴涔嬮棿鐨勬€ц兘杩涜浜嗗熀鍑嗘祴璇曘€傝鐮旂┒鍙戠幇锛孊GISEQ-500鍦ㄦ娴婻NA鍒嗗瓙鐨勭伒鏁忓害锛屽噯纭害鍜岄噸鐜版€ф柟闈笌Illumina骞冲彴鍏锋湁楂樺害鍙瘮鎬?/p>

铏界劧娴嬪簭璇曞墏鍜屼汉鍛樻垚鏈彈鍦板煙闄愬埗锛屼絾BGISEQ-500閫氬父鍏锋湁鏇撮珮鐨勬暟鎹悶鍚愰噺锛屼絾鎴愭湰鐣ヤ綆銆?ldquo;楂橀€氶噺鍜屾瘡閫氶亾鎴愭湰鐣ユ湁澧炲姞鐨勭粨鍚堜娇寰桞GISEQ-500鎴愪负scRNA-seq椤圭洰鐨勪竴涓湁鍚稿紩鍔涚殑鏇夸唬鏂规锛岄渶瑕佸ぇ閲忕殑澶氳矾澶嶇敤浠ュ強姣忎釜缁嗚優鐩稿綋澶х殑璇诲彇娣卞害”锛孊GI鐨勮仈鍚堜富瑕佷綔鑰呯吉鑻楄嫍鍗氬+璇淬€傜焊銆?/p>

“杩欐槸绗竴椤瑰皢Illumina HiSeq涓嶣GISeq-500娴嬪簭骞冲彴杩涜鍗曠粏鑳濺NA娴嬪簭姣旇緝鐨勭爺绌讹紝涓虹爺绌朵汉鍛樻彁渚涗簡鍙︿竴绉嶆祴搴忛€夋嫨銆傛垜浠殑鐮旂┒鍙戠幇锛屽钩鍙颁箣闂寸殑姣旇緝鎸囨爣琛ㄧ幇闈炲父鐩镐技銆傞€傜敤浜庡ぇ瑙勬ā鍗曠粏鑳炴祴搴忚鍒掞紝鐢熸垚鎵€鏈変汉绫荤粏鑳炵被鍨嬬殑鍙傝€冨浘璋憋紝澧炲己鎴戜滑瀵逛汉绫诲仴搴风殑鐞嗚В銆?ldquo;

锛堟湰鏂囨潵婧愶細浜掕仈缃?锛?

(璐d换缂栬緫锛氬ぇ鏋?

寮曠敤鍦板潃锛?a href="#" target=_blank>

TAG鏍囩锛?鍗曠粏鑳濺NA娴嬪簭 鏂囧簱鍒跺 娴嬪簭骞冲彴
椤朵竴涓?/div>
(1)
50%
韪╀竴涓?/div>
(1)
50%
鍏嶈矗澹版槑锛?/strong> 闄ゆ爣鏄庛€婂疄楠屽璧勮缃戙€嬪師鍒涘锛屾湰缃戦儴鍒嗘枃绔犺浆杞借嚜鍏跺畠濯掍綋锛岃浆杞界洰鐨勫湪浜庝紶閫掓洿澶氫俊鎭紝 骞朵笉浠h〃鏈綉璧炲悓鍏惰鐐瑰拰瀵瑰叾鐪熷疄鎬ц礋璐o紝涓斾笉鎵挎媴姝ょ被浣滃搧渚垫潈琛屼负鐨勭洿鎺ヨ矗浠诲強杩炲甫璐d换銆? 濡傚叾浠栧獟浣撱€佺綉绔欐垨涓汉浠庢湰缃戜笅杞戒娇鐢紝鑷礋鐗堟潈绛夋硶寰嬭矗浠汇€傚娑夊強浣滃搧鍐呭銆佺増鏉冨拰鍏跺畠闂锛? 璇峰湪30鏃ュ唴涓庢湰缃戣仈绯伙紝鎴戜滑灏嗗湪绗竴鏃堕棿鍒犻櫎鍐呭锛?
------鍒嗛殧绾?---------------------------
鍙戣〃璇勮
璇疯嚜瑙夐伒瀹堜簰鑱旂綉鐩稿叧鐨勬斂绛栨硶瑙勶紝涓ョ鍙戝竷鑹叉儏銆佹毚鍔涖€佸弽鍔ㄧ殑瑷€璁恒€?/small>
璇勪环:
琛ㄦ儏:
鏈€鏂拌瘎璁?/strong> 杩涘叆璇︾粏璇勮椤?gt;>